Phospho ire1 antibody
Web288 rows · IRE1 p Ser724 antibody (NB100-2323) antibody has been validated in … WebThe protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity …
Phospho ire1 antibody
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WebIRE1 alpha is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1 alpha is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities and has a theoretical molecular weight of 110 kDa. WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: …
WebPhospho-IRE1 alpha (Ser724) Polyclonal Antibody Advanced Verification This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. 29 Published Figures 63 References View all (42) IRE1 alpha antibodies Catalog # PA1 … WebWestern blot - Phospho-IRE1-S724 Rabbit mAb (AP1146) Western blot analysis of extracts of HeLa cells, using Phospho-IRE1-S724 antibody (AP1146) at 1:1000 dilution.Hela cells …
WebSeparation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody. Cell Counting Kit-8 (CCK-8) A convenient and sensitive way for cell proliferation assay and cytotoxicity assay. SYBR Safe DNA Gel Stain Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels. ...
WebSep 21, 2024 · The primary antibodies used in this study were as follows: XBP1 (Abcam, ab37152), ATF6 (Abcam, ab37149), phospho-IRE1 (Abcam, ab48187), ZIKV envelope protein (BioFront Technologies, BF-1176-56). Statistical analysis All experiments were reproducible and carried out in triplicate.
WebMay 7, 2024 · We generated an anti-phospho-S729 antibody to investigate such phosphorylation. Compared with pharmacological ER stress inducers or Toll-like receptor ligands, the bacterial subtilase cytotoxin has an unusual capability in causing rapid and strong phosphorylation at S729 and triggering B cells to express spliced XBP1. small things like these nedWebDescription. IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. small things lyrics ben howardWebMar 9, 2024 · IRE1α Antibody (B-12) is a mouse monoclonal IgG 1 κ IRE1α antibody, cited in 50 publications, provided at 200 µg/ml raised against amino acids 371-560 of IRE1α of … highway tarmac specificationWebthose that recognize nonphosphorylated IRE1. To characterize our anti–phospho-S729 antibody, we treated naive XBP1-proficient and -deficient B cells with LPS for a course of 72 h, immunopre-cipitated IRE1 using an anti–mouse IRE1 antibody, and showed that this antibody recognizes the 130-kD phospho-IRE1 protein small things like these plot summaryWebRabbit polyclonal antibody to IRE1 (phospho Ser724). (A258133) Buy rabbit polyclonal antibody to IRE1 (A258133). Applications: WB, ICC/IF and more. Reactivity: Human, … highway tallWebAug 23, 2024 · HEC-1-A cells were infected with HSV-1 (strain HF) at multiplicity of infection (moi) = 1. (A) Total cellular protein was harvested at indicated time point p.i. (post infection) and subject to Western blot analysis using antibodies against total IRE1, gD-1 (Glycoprotein D) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase). The number under ... small things like these word countWebIRE1 alpha (phospho Ser724) antibody Cat No. GTX130378 Datasheet File See all IRE1 alpha products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications. Calculated MW 110 kDa. ( Note ) Positive Control HeLa (100 nM Calyculin A, 30 min) Predict Reactivity small things like these questions