Phosphomotif antibodies

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WebNov 11, 2013 · Here we have used phosphomotif antibodies and mass spectrometry (MS) to investigate the effects of MEK/PI3K dual inhibition during the period immediately preceding cell death. Upon treatment, melanoma cell lines responded by dramatically increasing phosphorylation on proteins containing a canonical DNA damage-response (DDR) motif, … WebSep 20, 2009 · Using a phosphoproteomic approach combining the steroid receptor fusion system, IMAC, 2D-DIGE and phosphomotif-specific antibodies, we detected 38 proteins …

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WebFeb 11, 2013 · The scaffolding adapter protein Gab2 (Grb2-associated binder) participates in the signaling response evoked by various growth factors and cytokines. Gab2 is … http://www.phosphonet.ca/default.aspx city and states in japan

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WebAntiphospholipid antibodies are antibodies directed against phosphorus-fat components of your cell membranes called phospholipids, certain blood proteins that bind with … WebThe validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and … WebProtocols. As the epigenetics experts, Active Motif continues to develop new methods and helpful techniques to support your research. Our goal is to ensure your success with everything from gold standard assays to emerging approaches for epigenetics analysis. Check back often as we add new protocols, technotes and guides to this resource. city and state tax form

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WebPhosphospecific antibodies developed against key drug-regulated phosphorylation sites provide an ideal tool for this approach because they can be used for … WebJan 4, 2013 · These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase-induced substrate phosphorylation. This … city and suburban federal savings bankWebPhosphoproteomic screen for PDK4 substrates, followed by nonphosphorylatable and phosphomimetic mutations of the PDK4 site revealed cytoplasmic GTPase, Septin 2 (SEPT2), as the key effector … city and states in us

"Webby a recent study in which phospho-motif speciﬁc antibodies were used to enrich for peptides containing pS/pT-Q, with the goal of identifying substrates of SQ/TQ-directed kinases (e.g., ... including cell line and phosphospeciﬁc-versus-phosphomotif antibody differences. The phosphorylation sites identiﬁed in both data sets are of " - Phosphomotif antibodies

Phosphomotif antibodies

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WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies … WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and …

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WebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. Webnonphosphorylatable Ser172Ala mutation abolished interaction with the antibody to the 14-3-3 binding motif, whereas a nonphosphorylatable Ser155Ala mutation abolished the interaction with the antibody to the MPK substrate motif, and mutation of either site alone did not affect the AICAR-induced phosphorylation of the other site (Fig. 3C).

WebPhosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The … WebJan 1, 2005 · The antibody reactive with the phosphomotif generated by Akt, referred to here as the PAS (phospho Akt substrate) antibody, was purchased from Cell Signaling Technology, Beverly, MA (catalog number 9611). Rabbit antisera against peptides from the insulin-elicited phosphoproteins were generated for us by BioSource International, …

Webever, phosphomotif antibodies have not been used so far for large scale proteomics identification of kinase substrates. To discover novel MAPK targets at the synapse, we raised a phosphospecific antibody against a peptide library repre-senting the MAPK consensus phosphorylation motif. Using this antibody we affinity-purified putative MAPK ... WebATM-substrate phosphomotif blots showed a single, low molecular weight feature which similarly decreased in cells when PI3K was inhibited (i.e., in cells treated with PI3Ki alone …

WebOct 13, 2010 · Both studies 2,3 used phosphomotif-specific antibodies for immunoaffinity purification before MS analysis and quantified the effects of various pathway inhibitors on the newly identified Ser/Thr ...

WebPhosphoMotif Finder is especially useful for researchers who identify phosphorylation sites and would like to know if there is any specific enzyme associated with this function. The … dick sporting goods flatirons mallWebPhosphomotif Antibody Generation and Purification Phosphorylated and non-phosphorylated peptide libraries were chemically synthesized with C-terminal cysteine or … city and states of usaWebDec 8, 2015 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and suggest signaling pathways by which... city and state tax formsWebphosphomotif antibodies, indicating the pos-sibility of direct ERK substrates. From this screen, 38 candidate targets were identi-fied by mass spectrometry (MS), includ-ing 13 new substrates that were verified by in vitro ERK phosphorylation. Further stud-ies of one of them, the nucleoporin Nup50, revealed an unexpected role of ERK signal- dick sporting goods fire armsWebantibodies, generally crosslinked to a resin, targeting a consensus phosphomotif or a specific phosphoresidue, such as phosphotyrosine (pTyr) 13,14. This last option is highly specific but is generally limited due to the cost of use. IAP can be less efficient in complex samples due to the high background dick sporting goods football helmetsWebSep 29, 2014 · Atg9A bears a conserved canonical 14-3-3ζ binding phosphomotif. (A) HA-Atg9A was overexpressed in HEK-293 cells, and cells were treated with hypoxia for the indicated times. Immunoprecipitated HA-Atg9A was immunoblotted with an anti-14-3-3ζ phosphomotif antibody (pMotif) and for endogenous 14-3-3ζ. city and state taxesWebFeb 1, 2016 · Together, using a TNIK inhibitor and phosphomotif antibody, we identify endogenous substrates of TNIK in neurons, define consensus sequences for TNIK, and … dick sporting goods gainesville ga