Biotinylated mouse tim4-fc

WebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ... WebRecombinant Mouse TIM-4 (Catalog # 2826-TI) inhibits anti-CD3-induced proliferation of stimulated human T cells. Human T cells cultured for 72 hours with PHA are incubated …

PE Rat Anti-Mouse TIM-4 - BD Biosciences

WebMar 27, 2005 · The newly identified TIM family of proteins is associated with regulation of T helper type 1 (TH1) and TH2 immune responses. TIM-1 is genetically linked to asthma and is a receptor for hepatitis A ... WebMar 15, 2024 · Then concentrated CM were incubated with Streptavidin Magnetic Beads (60 mg) accompanied by additional 1 μg of biotinylated mouse Tim4-Fc, 350 μL Exosome Capture Immobilizing Buffer, and 50 μL Exosome Binding Enhancer overnight at 4 °C. The next day, the beads were washed with washing buffer for three times and the bound EVs … north fork vineyards map https://hssportsinsider.com

Tim-4 (human): FC (human) - Sigma-Aldrich

WebAug 31, 2024 · The EVs were isolated from the sera of PC patients or NC in cohort-1 using the Magcapture Exosome Isolation Kit (Fujifilm) according to the manufacturer’s protocol. In brief, 0.6 mg of streptavidin magnetic beads, bound with 1 µg of biotinylated mouse Tim4-Fc, was added to the serum supplement and rotated overnight at 4 °C. WebFeb 9, 2024 · Needle-like multi-walled carbon nanotubes (MWCNTs) are engulfed by macrophages, which cause inflammation and asbestosis-like pathogenesis in rodents; however, how macrophages recognize … Webadjective. bio· tin· y· lat· ed ˌbī-ə-ˈti-nə-ˌlā-təd. : combined with biotin. The PCR reaction uses biotinylated primers to define a sequence of approximately 207 nucleotides within … north fork virgin river

Tim4 recognizes carbon nanotubes and mediates ... - ScienceDirect

Category:APC anti-mouse Tim-4 antibody, Tim-4, RMT4-54 - BioLegend

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Biotinylated mouse tim4-fc

Tim4, a macrophage receptor for apoptotic cells, binds …

WebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … WebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express …

Biotinylated mouse tim4-fc

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WebMouse TIM4-Ig fusion protein Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. ... FC - Quality tested. Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg ... WebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM …

WebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, WebThis antibody was developed for use in flow cytometry. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with …

WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 … WebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching.

WebCD45 in Mouse Thymus. CD45 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse CD45 Biotinylated Monoclonal Antibody (Catalog # …

WebThe TIM4-affinity isolation method helps overcome the disadvantages of the affinity isolation method and enables the isolation of heterogeneous SEVs at high purity. This … north fork virginia navy baseWebTim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 … north fork volunteer fire departmentWebTIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by … north fork virginiaWebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these … north fork virgin river flow rateWebMouse TIM-4 Antibody Summary Species Reactivity Mouse Specificity Detects mouse TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with … how to say bottom in japaneseWebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. north fork vet southoldWebTIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich … how to say both of you in spanish